Changes and correlation of dengue fever epidemic and Aedes albopictus density in Haizhu district, Guangzhou before and after the COVID-19 pandemic

To analyze the population density, seasonal fluctuation of Aedes albopictus in Haizhu District, Guangzhou from 2017 to 2021, so as to provide a scientific basis for the monitoring and prevention and control of mosquito vector density of dengue fever. The data of dengue fever cases and Aedes surveillance data in Haizhu District, Guangzhou from 2017 to 2021 were collected, and the data of 2017-2019 and 2020-2021 were grouped to compare and analyze the characteristics of dengue epidemic and the density fluctuation of Aedes mosquitoes. A total of 517 dengue cases were reported in Haizhu District, Guangzhou from 2017 to 2021, of which only 7 cases were reported from 2020 to 2021, and the peak period of reported cases every year was August to November. Before the COVID-19 pandemic, there was a positive correlation between the number of local cases and the number of imported cases(rs=0.63, P<0.05) and BI(rs=0.73, P<0.05). The peak density of Aedes was from May to October, and the differences of mean BI(X~2=1 143.40,P<0.001), MOI(X~2=188.30,P<0.001), and SSI(X~2=4 499.43,P<0.001)before and after the COVID-19 pandemic were statistically significant. In general, before and after the COVID-19 pandemic, the density of Aedes in high-risk areas was higher than that in low-risk areas. After COVID-19 pandemic, the number of reported cases and the density of Aedes in Haizhu District decreased, but the density of Aedes in the high-risk area was still higher than that in low-risk areas, and a certain risk of outbreak still existed, so the government should continue to take more precise measures to strictly prevent dengue epidemic.

Disease threats to tigers and their prey

The contraction of the global tiger population over the last 100 years into small, often isolated subpopulations has made them increasingly vulnerable to the impact of disease. Despite this, the health of wild tigers continues to be insufficiently funded and explored. For example, canine distemper virus (CDV), has been associated with localized declines and increased risk of extinction, and yet has received little research attention in most tiger range countries. The emergence of new pathogenic threats has posed fresh challenges, including African swine fever virus (ASFV), which has the potential to devastate wild boar populations, and severe acute respiratory syndrome coronavirus (SARS-CoV2) with implications for tiger conservation that remain unknown. The objective of this review is to synthesize current research on the health of tigers and their prey that impacts the conservation of tigers in the wild. Published sources are interpreted based on three mechanisms through which disease can affect the viability of tiger populations: (1) by reducing the survival of adult tigers, (2) by reducing breeding productivity, and (3) by reducing the carrying capacity of tiger habitat through decreased prey abundance. Examples of CDV, SARS-CoV2, carnivore protoparvovirus 1 and ASFV are used to illustrate these processes and inform discussion of research and mitigation priorities. Copyright © 2023 Gilbert, Dvornicky-Raymond and Bodgener.

Plague God Image in the Clinic.

This paper selects four dreams before and during COVID-19 which constellated the Plague God image in Chinese culture. The author argues that this shows evidence that the origins of the modern psyche, although hidden, are preserved and living within the ancient anima mundi.

Cet article sélectionne quatre rêves produits avant et pendant la pandémie de COVID-19 et qui montrent la constellation de l'image divine de la peste dans la culture Chinoise. L'auteur soutient que ceci montre la preuve que les origines de la psyché moderne, bien que cachées, sont préservées et vivantes au sein de l'ancien anima mundi.

El presente artículo selecciona cuatro sueños antes y durante la pandemia del COVID-19 que constelan la imagen del dios de la peste en la cultura China. El autor argumenta que esto demuestra que los orígenes de la psique moderna, aunque ocultos, se conservan y viven dentro de la antigua anima mundi.

How do attitudes shape protective practices against the Asian tiger mosquito in community gardens in a nonendemic country?

BACKGROUND: The Asian tiger mosquito Aedes albopictus is responsible for the transmission of many arboviruses worldwide and is well adapted to thrive in urban environments. In mainland France, a nonendemic area, this mosquito is responsible for several autochthonous and imported cases of chikungunya and dengue each year. Better management and prevention of mosquito-borne disease transmission in nonendemic areas is thus of global concern. In this context, the aim of this study was to provide a better understanding of mosquito-human interactions as well as human behavior and beliefs in regard to this mosquito species in urban areas. METHODS: We focused on people who participate in community gardens, which are increasingly popular initiatives in metropolitan France and are conducive to the development of tiger mosquitoes. To evaluate community gardeners' knowledge and practices in relation to mosquito management and control, we conducted a knowledge, attitude, and practice (KAP) survey. RESULTS: In contrast to previous KAP studies, we showed that attitudes, more than knowledge, influence the practices of community gardeners in relation to mosquitoes. Interestingly, all gardeners who participated in the survey were concerned about the Asian tiger mosquito and were motivated to incorporate mosquito control methods in their gardens. Moreover, mosquitoes were perceived as nuisances rather than disease vector species. A change in community gardeners' perceptions could facilitate more appropriate behavior to control this species. CONCLUSIONS: This survey reveals the lack of knowledge and awareness of good practices for the efficient control of the Asian tiger mosquito in green urban areas.

COVID-19 lockdown and distressed reverse migration enhance human-tiger conflict in Sundarban: An eco-psychiatric observation.

COVID-19 lockdown enforced distressed reverse migration in Sundarban, which caused an unprecedented population addition to the already devastated fragile Sundarban ecosystem. Acute poverty and food crisis prompted many migrants to explore forest-based living, thus enhancing fatal human-tiger conflicts. Families of deceased migrants face significant mental health trauma and catastrophic psychosocial consequences.

Molecular Detection of Feline Coronavirus in Captive Non-Domestic Felids from Zoological Facilities.

Cases of feline infectious peritonitis (FIP), a disease with a high mortality rate caused by the feline coronavirus (FCoV), have been reported in non-domestic felids, highlighting the need for surveys of FCoV in these endangered species. With the aim of adding information on FCoV prevalence in captive non-domestic felids, samples (feces or rectal swabs and, when available, oral swabs, blood, and abdominal effusion) collected between 2019 and 2021 from 38 non-domestic felids from three different zoological facilities of Northern Italy were tested for evidence of FCoV infection via RT-qPCR. Three animals were found to be FCoV positive, showing an overall 7.9% FCoV prevalence ranging from 0% to 60%, according to the zoological facility. FCoV infection was detected in tiger cubs of the same litter, and all of them showed FCoV-positive oral swabs, with low viral loads, whereas in one animal, FCoV presence was also detected in rectal swabs at low FCoV copy numbers. Future studies should be carried out, including samplings from a higher number of captive non-domestic felids, in order to gain a deeper knowledge of FCoV epidemiology within these populations.

Potential Tiger-to-Human Transmission of SARS-CoV-2 at a Tennessee Zoo: A One Health Approach to Outbreak Investigation

Background. Human-to-feline and airborne transmission among cats of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been described, though documented feline-to-human transmission has not been reported. In October 2020, all 3 Malayan tigers at a Tennessee AZA accredited zoo were diagnosed with symptomatic SARS-CoV-2 infection. We investigated to determine source and prevent further transmission. Methods. Tiger nasal swab specimens were tested at the National Veterinary Services Laboratories (NVSL). An environmental assessment at the zoo was completed. We interviewed 18 staff who interacted with the tigers during the 2 weeks before animal symptom onset. Confirmed human cases were defined as persons testing positive for SARS-CoV-2 by RT-PCR during September 28-October 29, with tiger interaction during their 14-day incubation period. Interviewed staff had repeat SARSCoV-2 RT-PCR and serum IgG testing on October 29. Tigers and staff testing positive had specimens sent to CDC for genomic sequencing. Tiger sequences were compared phylogenetically with 30 geographically associated human cases collected within 2 weeks of the outbreak and > 200 background sequences from TN. Results. NVSL confirmed SARS-CoV-2 infection in all 3 tigers. Environmental assessment identified fencing between humans and animals allowing airflow and an open outdoor exhibit observation point above the habitat. Confirmed cases were identified in a tiger keeper and veterinary assistant;both developed symptoms after exposure to symptomatic tigers and one sample was genotyped. Staff did not report known contact with ill visitors. All staff were negative for SARS-CoV-2 IgG. The tigers and most temporally and geographically associated cases had genetic sequences in clade 20G and B.1.2. Tiger sequences were 3-6 single nucleotide polymorphisms different from the positive tiger keeper (Figure). Figure. Whole-genome phylogenetic analysis. Whole-genome phylogenetic analysis from a portion of clade 20G showing divergence estimates from SARS-CoV-2 Wuhan-Hu-1 reference genome with sequences from humans living in Tennessee and Malayan tigers sampled during the outbreak investigation in October 2020. Sequence analysis showed 3-6 single nucleotide polymorphisms (SNPs) differences between one human tiger keeper and all three tiger sequences. Differences are indicated by one-step edges (lines) between colored dots (individual SARS-CoV-2 sequenced infections). Numbers indicate unique sequences. Note not all analyzed sequences are shown in this figure. Conclusion. Using a One Health approach, we concluded the index tiger was likely infected via transmission from an ill visitor at an exhibit observation point or unidentified asymptomatic staff. Infection spread to the other 2 tigers and tigerto-human transmission to 2 staff is possible thereafter. The zoo was advised on infection control practices for humans and animals, and no additional cases were identified.

Investigation of SARS-CoV-2 infection and associated lesions in exotic and companion animals.

Documented natural infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in exotic and companion animals following human exposures are uncommon. Those documented in animals are typically mild and self-limiting, and infected animals have only infrequently died or been euthanized. Through a coordinated One Health initiative, necropsies were conducted on 5 animals from different premises that were exposed to humans with laboratory-confirmed SARS-CoV-2 infection. The combination of epidemiologic evidence of exposure and confirmatory real-time reverse transcriptase-polymerase chain reaction testing confirmed infection in 3 cats and a tiger. A dog was a suspect case based on epidemiologic evidence of exposure but tested negative for SARS-CoV-2. Four animals had respiratory clinical signs that developed 2 to 12 days after exposure. The dog had bronchointerstitial pneumonia and the tiger had bronchopneumonia; both had syncytial-like cells with no detection of SARS-CoV-2. Individual findings in the 3 cats included metastatic mammary carcinoma, congenital renal disease, and myocardial disease. Based on the necropsy findings and a standardized algorithm, SARS-CoV-2 infection was not considered the cause of death in any of the cases. Continued surveillance and necropsy examination of animals with fatal outcomes will further our understanding of natural SARS-CoV-2 infection in animals and the potential role of the virus in development of lesions.

“It Is a Tiger That Devours Me, But I Am the Tiger”

According to the Centers for Disease Control and Prevention, the animals known to have been infected with SARS-CoV-2 include otters, mink, white-tailed deer, dogs, ferrets, and felids, including domestic cats, lions, pumas, and tigers. Diagnostic samples taken from one tiger confirmed infection with SARS-CoV-2, and public health officials postulated that the source was exposure to a zoo employee positive for the virus. A 2014 literature review in PLoS One documents myriad cases in which humans transmitted influenza A virus, Mycobacterium tuberculosis, methicillin-resistant Staphylococcus aureus, and other pathogens to animals and stated that “transmission occurred in every continent except Antarctica therefore indicating a worldwide disease threat.” November 21, 2021 The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions.

The effect of COVID-19 pandemic on volatility transmission between the U.S. and emerging asia stock markets: A case study of tiger cub economies

The globalization in financial markets has highlighted the importance of a clear understanding of volatility transmission among equity markets in different countries. This paper looks into the effect of the COVID-19 pandemic on the volatility transmission between the U.S. stock market and five emerging equity markets called Tiger Cub economies in Southeast Asia. As the result of the dynamic conditional correlation GARCH (DCC-GARCH), the U.S. stock market’s volatility links positively to these smaller economies’ volatilities, and these linkages become stronger during the pandemic. We also find evidence of statistically significant co-volatility across five Tiger Cub markets. Due to the increase in financial globalization over the last few decades, the finding has relevant implications for policymakers, international investors, and portfolio managers. © 2021 by De La Salle University.

From People to Panthera: Natural SARS-CoV-2 Infection in Tigers and Lions at the Bronx Zoo.

Despite numerous barriers to transmission, zoonoses are the major cause of emerging infectious diseases in humans. Among these, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and ebolaviruses have killed thousands; the human immunodeficiency virus (HIV) has killed millions. Zoonoses and human-to-animal cross-species transmission are driven by human actions and have important management, conservation, and public health implications. The current SARS-CoV-2 pandemic, which presumably originated from an animal reservoir, has killed more than half a million people around the world and cases continue to rise. In March 2020, New York City was a global epicenter for SARS-CoV-2 infections. During this time, four tigers and three lions at the Bronx Zoo, NY, developed mild, abnormal respiratory signs. We detected SARS-CoV-2 RNA in respiratory secretions and/or feces from all seven animals, live virus in three, and colocalized viral RNA with cellular damage in one. We produced nine whole SARS-CoV-2 genomes from the animals and keepers and identified different SARS-CoV-2 genotypes in the tigers and lions. Epidemiologic and genomic data indicated human-to-tiger transmission. These were the first confirmed cases of natural SARS-CoV-2 animal infections in the United States and the first in nondomestic species in the world. We highlight disease transmission at a nontraditional interface and provide information that contributes to understanding SARS-CoV-2 transmission across species.IMPORTANCE The human-animal-environment interface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important aspect of the coronavirus disease 2019 (COVID-19) pandemic that requires robust One Health-based investigations. Despite this, few reports describe natural infections in animals or directly link them to human infections using genomic data. In the present study, we describe the first cases of natural SARS-CoV-2 infection in tigers and lions in the United States and provide epidemiological and genetic evidence for human-to-animal transmission of the virus. Our data show that tigers and lions were infected with different genotypes of SARS-CoV-2, indicating two independent transmission events to the animals. Importantly, infected animals shed infectious virus in respiratory secretions and feces. A better understanding of the susceptibility of animal species to SARS-CoV-2 may help to elucidate transmission mechanisms and identify potential reservoirs and sources of infection that are important in both animal and human health.